Buy Molecular Biology Homework Assignment Help

Buy Molecular Biology Homework Assignment Help
Buy Molecular Biology Homework Assignment Help
1. Which of the following elements is NOT required for a cell to synthesize
DNA?
(A) Phosphorus
(B) Nitrogen
(C) Hydrogen
(D) Carbon
(E) Iron
2. Which of the following DNA sequences is least likely to represent a
restriction endonuclease cut site?
(A) AGCT
(B) GACGAC
(C) GGATCC
(D) AAGCTT
(E) GATATC
3. In genetic engineering, the term “sticky ends” refers to
(A) the effects on mRNA following posttranscriptional modification
within the nucleus.
(B) the results on DNA following the work of the DNA replisome.
(C) the product produced by most restriction enzymes.
(D) the physiologic changes produced by an inversion mutation.
(E) the increased production of glycolipids following induction of the
glucose operon.
CHAPTER 2
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4. DNA ligase is used to
(A) initiate DNA synthesis in prokaryotes.
(B) stabilize the DNA helix to prevent supercoiling during DNA
replication.
(C) identify the site on an operon where RNA-polymerase binds to the
DNA helix.
(D) join together Okazaki fragments during lagging-strand DNA
synthesis.
(E) initiate DNA synthesis in eukaryotes.
5. If the base sequence triplet AAC was found on the DNA sense strand,
what would be the resulting amino acid added within the ribosome?
U C A G
Phe Ser Tyr Cys U
Phe Ser Tyr Cys C
Leu Ser STOP STOP A
Leu Ser STOP Trp G
Leu Pro His Arg U
Leu Pro His Arg C
Leu Pro Gln Arg A
Leu Pro Gln Arg G
Ile Thr Asn Ser U
Ile Thr Asn Ser C
Ile Thr Lys Arg A
Met Thr Lys Arg G
Val Ala Asp Gly U
Val Ala Asp Gly C
Val Ala Glu Gly A
Val Ala Glu Gly G
U
C
A
G
Second base in codon
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Third base in codon
First base in codon
(A) Leucine
(B) Asparagine
(C) Proline
(D) None; it codes for a halt to protein synthesis
(E) Glycine
Molecular Biology ‹ 13
Passage 2: Questions 48–52
While today site-directed mutagenesis has greatly streamlined research in genetic
expression, labs that are less well funded or reliant on older techniques can still produce advances within the field, albeit at a much slower pace. One such researcher
was utilizing near-visible ultraviolet light as a mutagen in investigating the efficiency of such light sources used in commercial bakeries. This researcher was utilizing Serratia marcescens, a coliform bacterium, as the reporter organism because of
its intense red pigment when grown under standard conditions.
The technique being used is replica plating, where organisms are irradiated and
grown on nutritionally complete agar medium, and then the respective colonies
are transferred via sterile cloth-covered plates that reproduce the exact same colony
location onto media that are deficient in one or more components. Mutants are
detected by their colonial presence on the complete medium but their absence on
the deficient medium. The colony present on the original medium is then used as
the source for mutant studies. In this case, the lab is studying lac operon expression.
The researchers used this older technique and observed the following.
48. One colony failed to grow on the replica plate that contained glucose as
the sole carbon source. What is the most likely cause?
(A) A point mutation in the beta-galactosidase gene
(B) A frame-shift mutation in the genome at a location other than the lac
operon
(C) An inversion mutation within the promoter region of the lac operon
(D) A deletion mutation of the lactose repressor gene
49. One colony failed to grow only on the replica plate that contained lactose
as the sole carbon source. What is the most likely cause?
(A) A point mutation in the beta-galactosidase gene
(B) A frame-shift mutation in the genome at a location other than the lac
operon
(C) An inversion mutation within the promoter region of the lac operon
(D) None of the above
50. One colony was discovered to have changed from inducible to constitutive
lac expression. What is the most likely cause?
(A) A point mutation in the beta-galactoside permease gene
(B) A frame-shift mutation in the genome at a location other than the lac
operon
(C) An inversion mutation within the operator region of the lac operon
(D) None of the above
14 › McGraw-Hill Education 500 MCAT: Biology
51. An extracellular material that was capable of passing through a 0.2-mm filter
was found to be able to change pigment expression from pigment negative
to pigment positive in species other than S. marcescens. The material was
possibly
(A) a phage.
(B) a DNA fragment.
(C) a plasmid.
(D) all of the above.
52. In order to help determine which material in the previous question was
responsible for the pigment change, which technique could eliminate one
of the possibilities?
(A) Protein precipitation
(B) Passage through a poly-T affinity column
(C) Passage through a poly-A affinity column
(D) Passage through a 0.1-μm filter
53. Which of the following mutations is most likely to produce a lethal
condition?
(A) A point substitution within the region coding for the leader segment
of the mRNA coding for a critical enzyme
(B) An inversion within a region coding for an intron within the mRNA
coding for a critical enzyme
(C) A point substitution within the TATAAT box within the promoter
for the gene coding for a critical enzyme
(D) A frame-shift mutation within a region coding for an intron within
the mRNA coding for a noncritical enzyme
(E) A silent mutation within a gene coding for a critical enzyme
54. Which of the following enzymes was discovered as a result of research into
why bacteria could survive infections with bacteriophages?
(A) β-lactamase
(B) DNA ligase
(C) DNA-dependent RNA-polymerase
(D) RNA-dependent DNA polymerase
(E) Restriction endonuclease
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